Institution: Universidade Federal de Goiás
In this work we developed a bovine serum albumin nanoparticle containing a near infrared IR-780 iodide multifunctional dye. The size distribution of the nanoparticles was obtained from nanoparticle tracking analysis. Fluorescence spectrophotometer was used to quantify IR-780 coupled to the nanocarriers. Photothermal properties were investigated at 808nm at distinct laser power. Healthy and tumor-bearing Swiss mice Ehrlich tumor models (breast cancer murine tumor model) were used for the in vivo studies. Acute oral toxicity testing and cardiovascular evaluation indicate the safety of the nanomaterial. Fluorescence molecular tomography (FMT) revealed the biodistribution of the nanocarrier and indicated the efficacy of nanocarrier accumulation in the tumor due to the EPR effect. The analysis of FMT data allowed us to extract information about the arrival and clearance nanocarrier time in the tumor region. In vivo tumor evolution studies showed several cases of total tumor regression due to the chemotherapeutic action of IR-780. Indeed, a very high survival rate for the treated group was found. A mathematical model was used to explain the data. The results indicate that the IR780-albumin-based nanocarriers have great potential for cancer imaging and therapy.
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