Universidade Estadual de Ponta Grossa (UEPG)OBJECTIVES: Aiming to improve the results of fat grafting, it is studied the reduction of estrogen signaling, a modulator of development and function of adipose tissue, which can be performed by the drugs Anastrozole and Exemestane, selective inhibitors of the aromatase enzyme. With this inhibition, it was expected that there would be a decrease in fat absorption rates and an improvement in graft survival.METHODOLOGY: Thirty female Wistar rats were weighed, scored, anesthetized and submitted to autologous fat grafting surgery, in which an inguinal fat cushion was removed and weighed in an analytical balance, being then implanted on the animal’s back. The rats were divided into three groups of 10 animals, group 1 defined as control, and groups 2 and 3, experimental, that received the drugs Anastrozole and Exemestane for 30 days. After this period, the grafts were removed, reweighed and prepared on histological blades containing hematoxylin and eosin. Inflammatory parameters, steatonecrosis, neovascularization and gigantocellular reaction were evaluated. Statistical analysis were performed using the Tukey test, with p<0.05.RESULTS: All rats obtained a mean weight gain of 38g during the survey. The comparison of the weight means of grafted fat and removed fat resulted in 63.2% of tissue absorption in the control group, and 62.8% to 62.85% in groups submitted to Anastrozole and Exemestane therapy respectively. The mean weight of the grafts was statistically equal among groups, with p=0.971 for implanted fat and p=0.983 for removed fat. The histological analysis showed similarity of the pre-established parameters, with variations from p=0.710 to p=0.965. No significant differences were observed.CONCLUSION: According to data analysis, it is concluded that the blockade of peripheral estrogen production by the drugs Anastrozole e Exemestane did not interfere in absorption and survival capacity of the grafts, and further research with animals ovarian hormonal deprivation is required.Absorption; Adipose tissue; Transplantation; Autologous; Estrogens; Aromatase.
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